FastQCFastQC Report
Wed 31 May 2023
EGAF00007868053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007868053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264829
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA58620.13744982506918801No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA51690.1212006389939667No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC51150.11993446865044297No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC44150.10352114938254266No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA43040.10091846589863275No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA42910.1006136471122289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)