FastQCFastQC Report
Wed 8 May 2024
EGAF00007868140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007868140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641847
Total Bases367.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC389871.0705282237282348No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC271600.7457754265898595No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG266040.7305084480484765No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG230330.6324538070929394TruSeq Adapter, Index 13 (97% over 37bp)
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC131680.36157477236138696No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC104350.2865304335959199No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA101670.2791715302702173No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA99510.27324047385845696No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT89710.24633105124954452No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC89500.24575442076506782No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT83090.22815346169127917No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT80840.22197527792902888No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT78940.2167581449742397No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC75090.20618658609216697No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC73770.20256205161831345No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC72120.19803138352599658No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG69550.19097452473978177No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA69170.18993109814882395No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG64180.1762292594938777No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG62790.17241251485853196No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT61770.16961173821964515No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT61700.1694195280581529No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG61300.16832118427819728No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC57100.15678857458866338No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG55910.15352100184329545No Hit
GAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAG55180.15151652444487645No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT54630.15000630174743748No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG52710.14473425160365058No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT49190.1350688263400412No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT48650.13358606223710112No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC47920.13158158483868213No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG47490.13040086527522984No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC46740.12834147068781307No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT44060.1209825673621105No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG43980.12076289860611937No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC42170.11579289300182023No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC42140.11571051721832357No Hit
CGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCA42090.11557322424582911No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA41830.11485930078885796No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC41440.11378841560340124No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA40860.11219581712246561No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG39630.1088184099991021No Hit
TCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGC39530.1085438240541132No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC39300.10791227638063873No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA38930.1068963083841798No Hit
GGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC38380.10538608568674082No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA38170.10480945520226412No Hit
GGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC38140.10472707941876745No Hit
GAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC37940.10417790752878964No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC36960.10148696526789841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)