FastQCFastQC Report
Wed 8 May 2024
EGAF00007868767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007868767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3911399
Total Bases395 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC927542.3713765841838175Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT199320.5095874903071765Illumina Single End PCR Primer 1 (96% over 33bp)
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG65530.16753596347496127No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG57170.14616253672918564No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA56470.14437289573372597No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC50760.129774538470762No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA48470.12391985578561533No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC47530.12151662359171232No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA47130.1204939715943068No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA43090.11016518642051093No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT41370.10576778283166713No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG39760.10165160854210986No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)