FastQCFastQC Report
Wed 8 May 2024
EGAF00007868770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007868770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3823164
Total Bases386.1 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG981722.5678207892729685TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT206890.5411486402362022TruSeq Adapter, Index 27 (97% over 39bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT128560.33626598283515957No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATGCTAAGTTGAGATGATATCAT89060.23294841654713216No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC85170.22277359799370364No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC79010.20666128892195051No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT71690.18751484372629582No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT61540.16096615264215713No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT59330.1551856001992067No Hit
GTGGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT56510.14780951065661846No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGG54990.14383374608047156No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA53310.13943947996999345No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT52840.1382101317128954No Hit
CCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGG51220.1339728036777915No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG49720.13004935179343602No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG47620.12455651915533836No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA47110.1232225455146575No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC46200.1208423180381485No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT44750.11704964788327155No Hit
GGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTT44040.11519254732467663No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA43920.11487867117392818No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCC43340.11336160311197742No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA42810.11197531677950515No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG41210.10779030143619264No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG40550.10606398260707622No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT39880.10431150743206413No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA39020.10206206168503365No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT38520.1007542443902485No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG38320.10023111747233444No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)