FastQCFastQC Report
Thu 9 May 2024
EGAF00007868775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007868775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3877274
Total Bases391.6 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC924712.3849488068163356Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT229320.5914464647069049Illumina Single End PCR Primer 1 (96% over 33bp)
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG64900.167385642593224No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG55040.14195540475086363No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA53560.13813828994288255No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC52150.13450171434879246No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA48060.12395306599430425No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA47310.12201871727404356No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC46620.12023911645140375No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA41640.10739504094887285No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT41280.10646655356314771No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTG41090.10597651855401502No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA39150.10097300319760738No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)