FastQCFastQC Report
Wed 31 May 2023
EGAF00007869939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007869939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3494510
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC61860.177020526482969No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC53060.15183816901368144Illumina Single End PCR Primer 1 (96% over 31bp)
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA50920.14571427753819563No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA48270.13813095398210337No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG45770.13097687515560122No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA45430.13000392043519693No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG39810.1139215512332201No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC36470.10436370192101324No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA35000.10015710357102998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)