FastQCFastQC Report
Wed 31 May 2023
EGAF00007871236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298699
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG719491.6737389614857892TruSeq Adapter, Index 6 (97% over 35bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT578831.3465236807694607No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT355630.8272968170137057No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT338280.786935768240577No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG330980.7699538860478484No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT311960.7257079409374789No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA287970.6699003582246629No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT279430.6500338823444024No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG269340.6265616643547268No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC268230.6239794877473394No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA249610.5806640567297221No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG206590.4805872660542178No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT201470.46867668566698895No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA198110.4608603672878701No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC183900.4278038541428465No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC173150.4027962879001297No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT170010.3954917522720246No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC169040.39323525559710043No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA153830.35785245722019615No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG152520.3548050235664325No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG138950.3232373329698125No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG138720.32270228736647993No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG119250.2774095139017642No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT115660.26905814991931276No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA114320.2659409277085928No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT108490.25237868480672876No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG105300.24495783491702955No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG104780.24374816659645163No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT104230.2424687097189173No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA101620.23639710526370886No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT101090.2351641740908121No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC98610.22939498671574818No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT98040.22806900413357623No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG95360.22183455971213614No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC94130.218973228876923No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA90030.20943545942621244No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT89670.20859799674273544No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA88470.20580645446447868No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC87470.20348016923259804No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG82000.19075538901421105No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG81720.19010402914928448No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT79610.18519556731001635No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC78430.1824505507363972No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT78170.18184571657610826No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC77600.1805197339939363No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC77600.1805197339939363No Hit
CACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTC77010.17914722570712674No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG75630.17593695208713148No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT74550.17342456403670042No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA73840.17177290152206517No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG73400.1707493360200377No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG72470.1685858907543887No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA71850.16714359391062272No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT71120.16544540569134988No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT70830.1647707829741045No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG70400.1637704803243958No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA69480.16163029791106565No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA68510.15937380123614145No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG68220.15869917851889606No Hit
CACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC68080.15837349858643276No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA67260.15646594469629066No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT66020.1535813510087587No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC65780.15302304255310736No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT63990.14885899198804103No Hit
GTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGT63780.1483704720893461No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT63480.1476725865197819No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT63010.14657923246079804No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT62620.1456719812203646No Hit
CCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG62030.144299472933555No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC61940.14409010726268576No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT61860.14390400444413531No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA61250.14248497045268813No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC60880.1416242449168923No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG60640.14106593646124096No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT60320.14032152518703914No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTC59600.13864659982008512No Hit
GGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTG57980.1348780177444385No Hit
CTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAA57280.13324961808212205No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT56380.1311559613734295No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG55790.12978345308661993No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG55740.1296671388250259No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG55070.12810852771966588No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT54670.12717801362691364No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGC54110.12587529389706048No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT53950.1255030882599596No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG53440.12431668279170047No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG53260.12389795144996196No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA51810.12052483786373505No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG51170.11903601531533146No Hit
CCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT50820.11822181548417324No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC50320.11705867286823293No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC49990.11629099874171232No Hit
CGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA49500.1151511189780908No Hit
CCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGAT49370.11484870189794633No Hit
CACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATT49340.11477891334098991No Hit
CTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGG48600.11305746226939827No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC48470.11275504518925376No Hit
GCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTAT48450.11270851948461616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48210.11215021102896482No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA47850.1113127483454878No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC47060.1094749830123021No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC46530.10824205183940536No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC46270.10763721767911641No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT45890.10675322929100176No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT45540.10593902945984354No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT44160.10272875583984828No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA44060.10249612731666022No Hit
GGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTT43760.10179824174709604No Hit
GTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATT43250.10061183627883694No Hit
ACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTT43140.10035594490333005No Hit
GTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTT43010.10005352782318558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)