FastQCFastQC Report
Wed 8 May 2024
EGAF00007871300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098307
Total Bases413.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTTCTTGGGTGGGTGTGGGTATAATGCTAAGTTGAGATGATATCAT224730.5483483789769775No Hit
GTGGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT135920.3316491419505664No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG127260.3105184653077478TruSeq Adapter, Index 8 (97% over 35bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT120260.2934382416934602No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG109940.2682571120221106No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT91780.22394613190275886No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC87510.21352719549804347No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA85230.20796392266367553No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT84830.20698790988571622No Hit
GGGTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG84610.2064511028578386No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG82640.20164423992638913No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATGCTAAGTTGAGATGATATCATT74250.18117237190869304No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA69530.1696554211287734No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG68670.16755699365616095No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA66350.16189611954399707No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA66020.16109090900218065No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT65410.15960248951579273No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT64550.15750406204318027No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA62550.15262399815338384No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT62230.1518431879310164No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT60850.14847594384705684No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT59690.1456455067909749No Hit
GGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTG58460.1426442674987501No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA57670.1407166422622805No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG56650.13822780967848433No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA55950.13651978731705555No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC55580.1356169754974432No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC55350.1350557681501166No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA55120.13449456080279004No Hit
GCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG51690.12612525123178914No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT51310.12519803909272778No Hit
GTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGT50850.12407562439807462No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT50290.12270920650893162No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA49580.12097678382805388No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT48950.119439563702768No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT47360.11555991291037981No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG46300.11297347904878771No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG46080.11243667202091011No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT45950.11211946786807332No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC44500.1085814215479709No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT43760.10677579790874622No Hit
GGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTT42580.10389656021376632No Hit
GAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACT41150.10040731453256185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)