FastQCFastQC Report
Wed 31 May 2023
EGAF00007871569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4599614
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC479451.0423700771412558Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC336470.7315179056329509No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC133270.2897417044125877No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT133050.28926340340732937No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT124070.2697400260108783No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT113790.24739032449244652No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT106820.23223687900767323No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG103750.22556240588884197No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTA101670.22104028729367292No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG100660.21884445086044177No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA96570.20995240035359491No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC87970.19125517923895352No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG83580.18171090008857266No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG73020.1587524518361758No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC72410.15742625359432336No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC71340.1550999714323854No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT70280.15279543022523195No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAA68150.14816460685614052No Hit
AAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCA65520.1424467357478258No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC59320.12896734378145644No Hit
TAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTT55270.12016225709374742No Hit
CTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGC55210.12003181136504062No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC54960.11948828749542896No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG54480.11844472166577458No Hit
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC54250.11794467970573183No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT54100.11761856538396484No Hit
TCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGT53530.11637933096125024No Hit
TACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG53070.11537924704116476No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC52110.11329211538185595No Hit
TGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTA51220.11135717040603843No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC51120.11113976085819376No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC50990.1108571284459957No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG50630.11007445407375488No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT49770.10820473196229075No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC48520.10548711261423242No Hit
TTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGC48450.10533492593074113No Hit
CTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAAC48010.10437832392022461No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC47630.10355216763841488No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC47330.10289993899488088No Hit
TAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGAC46950.10207378271307115No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA46430.10094325306427888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)