FastQCFastQC Report
Wed 8 May 2024
EGAF00007871577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4728939
Total Bases477.6 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC398010.8416475661876798Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC354610.7498722229235776No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC149770.3167095198309811No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT135120.2857300548812323No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT126620.2677556212926409No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT123850.2618980705820058No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT108960.23041109221328504No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG108900.2302842138585421No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTA105570.22324246517030563No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG104280.2205145805433312No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA99560.21053348330354865No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC93380.19746501276501982No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG83730.17705874404385424No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC82300.17403480992247944No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG79580.16828299117413018No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC79450.16800808807218703No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT75980.16067028988955026No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAA72410.15312102778234188No Hit
AAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCA68650.14516998421844732No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC63260.13377207868403462No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC63060.1333491508348913No Hit
TAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTT61200.12941592183785836No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG58770.12427734847076692No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT57430.12144373188150662No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG56700.11990004523213346No Hit
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC56340.11913877510367549No Hit
TACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG56190.11882157921681799No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC55930.11827177301293167No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC55710.11780655237887398No Hit
CTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGC55390.11712986782024468No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC55220.11677037914847284No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA54450.11514210692927104No Hit
TGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTA54220.1146557399027562No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC53740.11364071306481222No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC52470.11095512122275208No Hit
TCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGT52430.1108705356529234No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT52180.11034187584149426No Hit
CTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAAC51490.10888277476194977No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT51260.10839640773543495No Hit
TTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGC50610.10702189222571913No Hit
TAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGAC49910.10554164475371748No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT49390.10444203234594483No Hit
GGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAA48560.10268688177200003No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC48450.1024542714549712No Hit
CTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGC48390.10232739310022819No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG48340.10222166113794234No Hit
GGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGC48100.10171414771897036No Hit
ATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGACC47910.10131236626228421No Hit
CGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGA47440.10031848581679738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)