FastQCFastQC Report
Wed 8 May 2024
EGAF00007871579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4651607
Total Bases469.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC378490.8136757898936862Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC332120.7139898104031575No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC142830.3070551746955407No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT131590.2828914824489687No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT118920.2556535838044788No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT118210.2541272295789391No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT105030.22579293564568118No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG102870.22114937912854632No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTA99690.2143130320338756No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG98210.2111313359017647No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA93760.2015647495585934No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC88780.190858772032977No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG79490.17088717941992948No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC76830.16516872556086531No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC76680.1648462563582865No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG76150.16370686517584138No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT71320.15332335685280377No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAA69920.15031364429540156No Hit
AAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCA67750.14564858983142814No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC60540.13014857016080678No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC60080.1291596646062318No Hit
TAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTT56980.12249530108626977No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG56450.12135590990382462No Hit
TACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG55560.11944259263519039No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT54900.11802372814384361No Hit
CTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGC54470.11709931642978437No Hit
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC54190.11649737391830393No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG53760.11557296220424469No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC53100.11415409771289792No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC53050.114046607978705No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC52640.11316519215832292No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC52300.11243426196581095No Hit
TGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTA51950.1116818338264604No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC50480.10852163564118808No Hit
TCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGT50380.1083066561728022No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA50370.10828515822596363No Hit
CTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAAC50120.10774770955499895No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT48680.1046520052102424No Hit
TTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGC48540.10435103395450218No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT48260.10374909144302173No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT48120.10344812018728151No Hit
TAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGAC47900.1029751653568326No Hit
CTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGC47020.10108334603503692No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC46610.10020193021465484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)