FastQCFastQC Report
Wed 8 May 2024
EGAF00007871584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4121464
Total Bases416.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG368380.8938086078150871TruSeq Adapter, Index 19 (97% over 37bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC197560.4793442330201113No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT174170.4225925544903461No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC140450.3407769666312747No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG137980.33478395055737475No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT137300.3331340513953294No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT123410.2994324346882564No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA110360.2677689287107688No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC103540.251221410644373No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC102860.24957151148232762No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT100440.24369981152328393No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG96600.23438273390232206No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG95550.23183509549034031No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT78860.19133977635131594No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT77340.1876517664596852No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT74480.1807124846899063No Hit
GGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATC74450.18063969502099253No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG71300.17299677978504727No Hit
TTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTC67470.16370396538705664No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT67150.16292754225197648No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA66620.1616415914345No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG66280.1608166418534773No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT65680.15936084847520202No Hit
CGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA65650.15928805880628824No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC64550.1566191042794502No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT64130.1556000489146575No Hit
CTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGT63890.15501773156334742No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT63650.15443541421203727No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC62930.1526884621581069No Hit
GGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG62520.15169367001628548No Hit
ATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT61300.14873355681379238No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT61030.14807844979356852No Hit
GCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTA59480.14431765023302398No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG57410.13929516307797424No Hit
TCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC56210.13638357632142364No Hit
GGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGG54890.133180830889218No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG54090.13123977305151763No Hit
GGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACT53050.12871639786250713No Hit
CGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACC52760.1280127643963407No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC52620.12767307927474314No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA51860.1258290743289278No Hit
TCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTC51710.12546512598435897No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC49670.12051542849822296No Hit
CGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTG49590.12032132271445292No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT49470.12003016403879788No Hit
GGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAG49300.11961768924828653No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA48960.11879273966726386No Hit
CTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAG48850.11852584421458007No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA48680.11811336942406873No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT48030.11653625993093715No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC47510.11527457233643192No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT46360.1124843016947376No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA45310.10993666328275584No Hit
TCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGA44550.10809265833694047No Hit
CTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACT44540.10806839511396922No Hit
TAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAA44300.10748607776265909No Hit
CCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAG44200.10724344553294654No Hit
TTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA43900.1065155488438089No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT42550.10324001374268948No Hit
TTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGTTGACAG42420.10292459184406318No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG42000.10190553647927048No Hit
CTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCT41570.10086221789150651No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG41560.10083795466853526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)