FastQCFastQC Report
Thu 9 May 2024
EGAF00007871586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221695
Total Bases426.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG373080.8837208751461202TruSeq Adapter, Index 19 (97% over 37bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC200780.47559096524026484No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT177980.42158422150344826No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC142890.338465947919023No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG142820.3383001377408837No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT141420.3349839341780967No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT124800.2956158604541541No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA114090.27024690319883365No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC106670.25267102431606264No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC105370.24959169243633184No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT100880.2389561538671079No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG98450.23320017196884188No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG94320.2234173714586203No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT80550.19080014070177975No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT80470.19061064335533476No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT77410.18336236985381463No Hit
GGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATC77310.1831254981707584No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG72990.17289264146273No Hit
TTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTC69820.16538380910984807No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT69550.1647442555655963No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG68560.16239922590333977No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC68200.1615464878443374No Hit
CGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA67250.15929620685530338No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA66940.1585619046378291No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT66310.15706961303457498No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT65820.15590894178759954No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC65680.15557732143132083No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT64970.15389553248162172No Hit
CTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGT64650.15313754309584185No Hit
ATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT63930.1514320669778371No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT63910.15138469264122587No Hit
GGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG63790.1511004466215584No Hit
GCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTA61180.14491809569379124No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG58850.13939898547858146No Hit
TCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC58820.13932792397366459No Hit
GGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGG57580.13639071510376755No Hit
CGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACC57440.13605909474748884No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG56060.13279026552131312No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC55500.13146378409619833No Hit
GGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACT53640.12705797079135278No Hit
TCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTC53210.12603942255421105No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT52970.12547093051487615No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA52800.12506824865368057No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC50760.12023606631933384No Hit
CGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTG50690.12007025614119449No Hit
CTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAG50650.11997550746797199No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA50280.119099082240664No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC50210.11893327206252466No Hit
GGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAG49890.11817528267674476No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT48520.11493014061887465No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA48020.11374578220359358No Hit
TAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAA46910.11111650652166961No Hit
CCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAG46240.10952946624519298No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT46000.10896097420585807No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA45940.10881885119602434No Hit
TCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGA45650.10813192331516133No Hit
CTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACT44990.10656857020699032No Hit
CTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCT44850.10623694985071164No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT44840.106213262682406No Hit
TTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA43940.10408141753490009No Hit
GGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG43700.10351292549556518No Hit
TTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGTTGACAG43290.10254175159503469No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG42730.10121527016991991No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT42640.1010020856551693No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG42630.1009783984868637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)