FastQCFastQC Report
Mon 22 May 2023
EGAF00007871591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160951
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC379060.910993664669447Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC305020.7330535735700805No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC127860.30728552198764175No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT117670.28279592814238863No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT108700.26123835632767606No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT107500.25835440023206235No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT92440.22216075123211018No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG91800.2206226413144495No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG88910.21367711371751313No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTA87190.20954344331380015No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA84520.20312664100105962No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC77630.18656792641874417No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG75830.18224199227532362No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC74770.17969449772419815No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC68880.16553907988822747No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG67060.16116507980988No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT65340.157031409406167No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAA62060.14914859607815617No Hit
AAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCA57860.13905474974350818No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC54260.130402881456667No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC53900.12953769462798287No Hit
TAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTT52790.12687003523954019No Hit
CTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAG51030.12264023296597341No Hit
TACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGG50210.12066952963397069No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC50180.12059743073158036No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT49940.12002063951245762No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG48870.11744911199386872No Hit
CTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGC48780.11723281528669767No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC48570.11672812296996528No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC47400.11391626577674191No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC47320.11372400203703432No Hit
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC46890.11269058443610608No Hit
CTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC46740.11233008992415437No Hit
TGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTA46460.11165716683517782No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT46260.11117650748590888No Hit
TCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGT44770.10759559533385517No Hit
TAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGAC44740.10752349643146483No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT44500.10694670521234208No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA44460.10685057334248829No Hit
TTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGC44410.10673040850517106No Hit
CTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAAC44090.10596135354634074No Hit
CAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACT42590.10235640842682359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)