FastQCFastQC Report
Mon 22 May 2023
EGAF00007871594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4117219
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG365260.8871522258106745TruSeq Adapter, Index 19 (97% over 37bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC202670.4922497443055616No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT177170.43031473429030614No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC143310.3480747562857356No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG137880.33488624238836945No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT136810.33228740079165087No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT121000.2938876945821925No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA110080.2673649373521302No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC107220.2604185009347329No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC105820.2570181474436993No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT100840.2449226043113082No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG97790.2375146913486992No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT79120.19216854872184355No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT78490.19063838965087843No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG77400.18799097157571654No Hit
GGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATC76860.1866794066577464No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT76310.18534355350055462No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG71420.17346660452115858No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT69410.16858466843760314No Hit
TTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTC67240.16331412052650102No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG67000.16273120278518097No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA66940.16258547334985093No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC66700.1620025556085309No Hit
CGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA66310.16105531427888584No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT65900.1600594964707974No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC65690.15954944344714234No Hit
CTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGT64650.1570234665680888No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT64160.15583334284622702No Hit
GGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG63730.15478894855969527No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT63110.1532830777279518No Hit
GCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTA60310.14648237074588455No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT58430.1419161817722108No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG58310.1416247229015508No Hit
ATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT57730.14021600502669399No Hit
TCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC57270.13909874602249722No Hit
CGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACC57050.1385644047596205No Hit
GGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGG56340.136839939774882No Hit
CCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAG54730.13292953326019336No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC54700.13285666854252834No Hit
GGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACT53170.12914056794161302No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA51290.12457437896793927No Hit
GGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAG50830.12345711996374252No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA50520.12270418454787077No Hit
TCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTC50390.12238843743798909No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC50300.12216984328499408No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT50270.12209697856732907No Hit
CGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTG50010.12146548434756568No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC49500.12022678414726057No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA49270.11966815464516219No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT48060.11672927769934027No Hit
CTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAG47200.11464048912627674No Hit
TCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGA46570.11311033005531161No Hit
TACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACA45980.11167732394123314No Hit
TAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAA45470.11043862374092803No Hit
CCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAG45410.11029289430559804No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT45310.11005001191338135No Hit
CTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACT44860.10895704114840625No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT44670.10849556460319452No Hit
CTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCT43080.1046337345669492No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG42400.10298213429987572No Hit
TTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA42090.10222919888400399No Hit
GATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGC41930.1018405870564573No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC41910.10179201057801394No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC41720.10133053403280225No Hit
GGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG41690.10125766931513723No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT41550.10091763396603388No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG41500.10079619276992552No Hit
TTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGTTGACAG41450.1006747515738172No Hit
CCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTT41240.10016469855016213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)