FastQCFastQC Report
Mon 22 May 2023
EGAF00007871742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329622
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT205340.6167066411742835No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC166920.5013181676478591No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT144470.4338930965737252No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT89770.26961018397884207No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA81060.24345105840843195No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG75470.22666236587816874No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA72030.21633086278262217No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA64090.19248431203301755No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT63660.19119287414607425No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG61080.1834442468244143No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA57240.1719114061596181No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT54180.16272117375485867No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT53910.16191027089561516No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT53750.16142973586791534No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC52770.1584864588232538No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCGATTGAGTATA52220.1568346196655356No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG51730.15536298114320485No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT50540.15178900187468727No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG48600.1459625146638267No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC48090.14443080926303348No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT44600.13394913897133068No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA42550.12779228392892647No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG41850.12568994318273966No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA41720.12529950847273355No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC41610.1249691406411899No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA40990.12310706740885301No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCG39130.11752084771184236No Hit
CTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCG38590.1158990419933554No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT38100.11442740347102465No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC37940.1139468684433248No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG37180.11166432706175054No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG36960.11100359139866328No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA36520.1096821200724887No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA35170.10562760577627131No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT35070.1053272713839589No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA34210.10274439561007226No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG33570.10082225549927289No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT33460.10049188766772925No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA33380.10025162015387933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)