FastQCFastQC Report
Thu 9 May 2024
EGAF00007871972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007871972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5086656
Total Bases381.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT340020.6684548748726079No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT269000.528834660727991No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC242760.477248707205677No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA229310.4508069741692774No Hit
GGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACC223130.4386575384692812No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA206930.406809503139194No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC181020.35587230589212243No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT172440.339004642735817No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT135750.2668747404974899No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA129150.2538996149926395No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA126920.24951559531448558No Hit
GGGCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTAC123120.24204506850866264No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG113630.2233884107751733No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT111010.21823767913536912No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG98460.1935652813950855No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT89710.17636341046062481No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA85760.16859799443878257No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG80570.1583948275645139No Hit
GCCGTGAGTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCT73350.14420082663345035No Hit
GCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGA73030.14357172963927578No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA72480.14249046918053826No Hit
GTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCACCTCAGCCATT71090.1397578291120925No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT65100.12798191975238743No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT61790.12147469771889431No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC61230.12037377797908883No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC61050.12001991091986564No Hit
GGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCACCT58790.11557691339850779No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT56260.11060311528831517No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT55200.10851923149511192No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC54460.10706444469608324No Hit
GAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCAC53870.1059045471130739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)