FastQCFastQC Report
Fri 10 May 2024
EGAF00007872687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007872687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830638
Total Bases386.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC204660.5342713145956366Illumina Single End PCR Primer 1 (96% over 32bp)
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG105460.27530661994163896No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC91470.2387852885080762No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC78160.20403911828786744No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC63450.1656382043931063No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC57830.1509670190709746No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT55210.1441274273371694No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC53260.13903689150475718No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG49890.13023940137387036No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT47140.12306044058457102No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG46670.12183349092239988No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT44350.11577705854742734No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG42100.10990336335618245No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT40970.10695346310457944No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC39440.10295935037453292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)