FastQCFastQC Report
Thu 9 May 2024
EGAF00007872966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007872966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629405
Total Bases366.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT264790.7295686207518863No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT204840.564390030872829No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT176110.48523105026857016No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG160590.44246922016143136TruSeq Adapter, Index 7 (97% over 35bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG157380.43362479524880804No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA155700.4289959373506126No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG147630.4067608878039238No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT125050.3445468334341304No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA123040.33900873559164657No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA117870.3247639764644618No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT113480.3126683299328678No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG112950.31120803547688947No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC94980.26169578760154905No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT85520.23563090919861523No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC82000.2259323497928724No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG80220.22102796463883198No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT79070.21785940119661487No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA75530.20810573633970306No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG70460.19413650446836328No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC67540.18609110859769024No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT60710.16727259702347907No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC52000.14327417303938247No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC49290.1358073844059839No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT49130.13536654079663196No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT48680.13412666814532961No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG48170.1327214791405203No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG47400.13059991927051404No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT47350.13046215564259156No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC46920.1292773884424582No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG44810.1234637633441294No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC42000.11572144745488584No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG41640.11472954933384398No Hit
CACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC41190.11348967668254163No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC40070.110403771417078No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA39420.10861284425408572No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT38880.10712499707252289No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG38800.10690457526784693No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC38530.10616065167706552No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT37980.10464525176991822No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT37850.10428706633731975No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCC37050.10208284829056001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)