FastQCFastQC Report
Mon 22 May 2023
EGAF00007874829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007874829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943024
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC137620.3490214617004614Illumina Single End PCR Primer 1 (96% over 33bp)
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG83270.2111830919619054No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC67580.17139129764363595No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG59550.15102621744123293No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA53930.136773197424109No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA51080.12954524243321877No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT47380.1201615815678525No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT42090.10674548265493691No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT40550.10283985083529798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)