FastQCFastQC Report
Wed 8 May 2024
EGAF00007874946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007874946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4642271
Total Bases468.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT131670.2836327306182685No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT108990.23477733204287296No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA102640.2210986820890034No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA91520.19714488878395942No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT90750.19548621784467127No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT83250.17933033207238439No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG79970.1722648246946376No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA77420.16677182353206005No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG75570.16278670504156265No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG68650.14788020776899927TruSeq Adapter, Index 6 (97% over 35bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG67810.14607074856250313No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC65120.1402761708655096No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA64800.13958685307255866No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT64010.1378850997712111No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT58400.12580049721354053No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC56390.12147071982656764No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG52410.11289732977674075No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG51060.1099892703377291No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT50240.10822289349329241No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC48830.10518558696810247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)