FastQCFastQC Report
Wed 31 May 2023
EGAF00007875849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007875849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC269830.7589362685356887Illumina Single End PCR Primer 1 (96% over 31bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT53990.15185475721099148No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG51850.14583569478403238No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG46610.1310974297759643No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG43850.12333452683278343No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC38090.10713368590788415No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA37720.1060930068901389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)