FastQCFastQC Report
Wed 8 May 2024
EGAF00007876289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007876289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581449
Total Bases361.7 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC326980.9129824269450717Illumina Single End PCR Primer 1 (96% over 33bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC116400.3250081182225406No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG87030.24300220385659546No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG75690.21133904182357474No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT75620.21114359020608697No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG74410.20776506938951245No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG71800.2004775162231823No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT71570.19983531805143673No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT68330.19078870032771653No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA65980.18422711031205527No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA64390.1797875664291185No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG63700.17786097191388178No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT60920.1700987505336527No Hit
AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT60690.16945655236190715No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT56490.1577294553126402No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC55950.1562216856920202No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG53670.14985554729384673No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG52220.14580690664588553No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA51980.14513678681449882No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC50840.1419537176154121No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT50670.1414790494015132No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA49680.13871480509704312No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT49410.1379609202867331No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA49190.13734664377462866No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA48590.13567134419616195No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT47250.13192984180425296No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG47070.13142725193071297No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA45270.12640135319531284No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG44720.1248656619150517No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT44670.12472605361684615No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC44430.12405593378545945No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA43860.12246439918591609No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA43830.12238063420699276No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG43520.12151506275811828No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC42980.12000729313749825No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGAC42360.11827615023974933No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG42240.11794109032405599No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT40780.11386452801645368No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT39660.11073730213664915No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT39610.11059769383844359No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT38880.10855941268464245No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA38290.10691203476581686No Hit
GGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTAT37640.10509712688914459No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC37590.10495751859093903No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG37390.10439908539811679No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT35940.10035044475015559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)