FastQCFastQC Report
Wed 8 May 2024
EGAF00007876309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007876309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312525
Total Bases334.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC1330564.016754590531392Illumina Single End Sequencing Primer (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT83070.2507754658455408Illumina Single End Sequencing Primer (100% over 32bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT71880.21699458872008515No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG60320.18209673889253666No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG59020.17817224020950784No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC54790.16540252526396027No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG45560.13753858461445576No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA41690.12585565391959305No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG40340.12178021297952468No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG37130.11208972007758433No Hit
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTTAAGATTAGTGTAGATCTC35800.10807465604033177Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)