FastQCFastQC Report
Wed 31 May 2023
EGAF00007876714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007876714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032482
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT376900.9346600927170908No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT267300.6628671870079024No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG240000.5951669468084421TruSeq Adapter, Index 2 (97% over 36bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT209940.5206222867206847No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA198810.4930214195624432No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG195070.483746734641345No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT192210.476654328525211No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG180960.4487558778935653No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC163550.4055814756271696No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA155330.38519700769898046No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA148920.36930109049463833No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT140840.3492638032854207No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT137610.34125384812629045No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC136630.3388235830934893No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC130330.3232004507397677No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG126010.31248744569721576No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG107180.2657916389955367No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC99610.24701908154828714No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA94500.23434698530582407No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT93130.23094957398445917No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG85980.21321855869412434No Hit
CACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC68520.1699201633138102No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG67540.16748989828100908No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT64240.159306352762393No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT63800.15821521335991084No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT59930.1486181463426247No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG59200.1468078468794157No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA58450.14494795017063933No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG58400.1448239570567209No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC57490.14256728238340555No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG53660.13306940985725418No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT53310.1322014580598252No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC52310.12972159578145667No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC52270.12962240129032193No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG51820.1285064632650561No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA51510.12773770595876188No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT50030.12406750978677648No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG45910.11385047719989824No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA45470.11275933779741608No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT45330.1124121570784445No Hit
CACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTC44390.11008108653677809No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC43000.10663407796984586No Hit
CCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG42920.10643568898757638No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC42890.10636129311922533No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG42600.10564213305849846No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG42270.10482377850663685No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT42270.10482377850663685No Hit
CCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT42180.10460059090158368No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC41570.10308787491177888No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT41510.10293908317507679No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT41380.10261670107888889No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG40780.10112878371186779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)