FastQCFastQC Report
Wed 8 May 2024
EGAF00007876931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007876931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4018882
Total Bases405.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG179400.44639280277450294No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC151170.3761493868195184No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT96380.23981793941698212No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT96060.23902169807424054No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC81660.20319083765086907No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT81030.20162323750734656No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG77320.19239181443993628No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT77230.1921678715622902No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA72040.17925383228470007No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA69040.17178906969649768No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT68590.17066935530826732No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG67350.16758392010514367No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT64380.1601938051428233No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA64170.15967127176164914No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTG62670.15593889046754794No Hit
AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT62630.15583936029970524No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA61450.15290322034834564No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA59890.1490215438024804No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA59740.14864830567307027No Hit
GGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT59030.14688164519386238No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC57350.14270137814446904No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT57080.14202954951153082No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGAC57050.1419549018856488No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT56590.14081030495545777No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC55900.1390934095601712No Hit
CCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGA55280.1375506919586094No Hit
TGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACT54860.13650562519626103No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC54610.1358835616472442No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA54010.1343906091296037No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG52180.12983710395080025No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG50790.12637843061826648No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC50030.1244873574292552No Hit
GGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTAT48420.12048126817358659No Hit
CCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA47960.11933667124339556No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC47800.11893855057202476No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG47760.11883902040418207No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG47730.11876437277830003No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC47510.1182169568551652No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT46860.11659959162772134No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC46750.11632588366615393No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT45780.11391227709596848No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCC43700.10873670836814815No Hit
GAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACA43240.10759211143795712No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC43220.10754234635403578No Hit
GCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTG42600.10599962875247394No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA42290.10522826995169304No Hit
GTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGA42250.10512873978385034No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA42210.10502920961600765No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG42030.10458132386071549No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG41550.10338696184660312No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG40980.10196865695484465No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC40970.10194377441288399No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG40660.10117241561210306No Hit
GGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCT40590.10099823781837836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)