FastQCFastQC Report
Thu 9 May 2024
EGAF00007878461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4206545
Total Bases424.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC136650.3248509168450593Illumina Single End PCR Primer 1 (96% over 32bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT73460.1746326260624812No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG69330.164814592498119No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG66970.15920428760419775No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG59620.141731516006604No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA59540.14154133617969142No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC48760.11591460450322058No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG48690.11574819715467206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)