FastQCFastQC Report
Mon 22 May 2023
EGAF00007878618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180610
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC200000.628810196786151No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG149770.47088451586330926No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC148590.46717453570227097No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG120080.37753764215040514TruSeq Adapter, Index 7 (97% over 37bp)
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT108210.34021775697114703No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG104740.32930790005690735No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT86910.27324947101342195No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA84960.267118571594757No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT83830.26356579398291524No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC82380.2590069200562156No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC80780.2539764384819264No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG77090.24237489035122192No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG63400.1993328323812099No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT63150.1985468196352272No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA61750.19414514825772414No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT59920.18839153495713087No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT58580.18417850663866364No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG54990.17289136360635224No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC54770.17219967238988748No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG53720.16889841885676019No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT51080.160598124259183No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA50730.15949770641480723No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA46080.1448778693395292No Hit
GCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTA45180.14204822345399154No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG44510.13994170929475794No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG44100.13865264839134633No Hit
TCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCAC43610.13711206340922022No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT39390.12384416825703246No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT38160.11997698554679763No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT36660.11526090907090149No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC36610.11510370652170496No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC36530.1148521824429905No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC36450.11460065836427603No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT36410.1144748963249188No Hit
TAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCC36020.11324871644118582No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC34300.10784094874882492No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT34030.1069920549831636No Hit
CTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGT33770.10617460172734161No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33670.10586019662894855No Hit
TTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTC32930.10353359890083978No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT32710.10284190768437501No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG32440.10199301391871371No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG31850.10013802383819455No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA31830.10007514281851594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)