FastQCFastQC Report
Wed 8 May 2024
EGAF00007878619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180610
Total Bases321.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC137520.4323698913101575No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC124670.3919688361666473Illumina Single End PCR Primer 1 (96% over 32bp)
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT95590.3005398335539409No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC92880.2920194553874886No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC85210.26790458434073966No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG70190.2206809385620997No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT62920.19782368790892316No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC62280.19581149527920744No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG61250.19257312276575878No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT54910.17263983952763778No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC52270.1643395449300606No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC50980.16028371916078993No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG50170.157737037863806No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC49940.15701390613750194No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG45640.14349448690659966No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC44990.1414508537670447No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT44650.1403818764325082No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT44270.13918713705861455No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC44250.13912425603893594No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT43400.1364518127025948No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG43060.13538283536805834No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG42770.1344710605827184No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA41530.1305724373626443No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG41530.1305724373626443No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA41480.13041523481344774No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC41440.1302894727740905No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC37410.11761894730884957No Hit
CCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCC37240.11708445864158133No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT35660.11211685808697075No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC35080.1102933085162909No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC34760.10928721220143306No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATTACCCTATAGAAGAACTA33440.10513706490264446No Hit
AAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCT33420.10507418388296585No Hit
CGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGA32610.10252750258598192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)