FastQCFastQC Report
Mon 22 May 2023
EGAF00007878623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786957
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC116120.4166551547081638No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC115890.4158298818388658Illumina Single End PCR Primer 1 (96% over 32bp)
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC83300.29889230440225667No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT83170.2984258458239578No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC73360.26322616387694536No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG62570.22451010187814163No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT55840.2003619000939017No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC54830.19673787575481072No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG52450.18819809562903195No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC46050.16523398100508907No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT45160.162040533815197No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG42610.15289076939471977No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC41490.14887204933552975No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC40170.14413570069434153No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT39850.1429874949631444No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC39120.14036815063885089No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG39070.14018874349335136No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT38600.13850231632565554No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG37570.13480652912836474No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG37450.1343759519791658No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC37080.1330483391024691No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG36690.1316489633675726No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC36670.13157720050937277No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT36170.12978312905437722No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA36060.1293884333342782No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA34020.12206862179789642No Hit
CCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCC32120.11525115026891337No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC30480.109366595896528No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT30060.10785957587433175No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATTACCCTATAGAAGAACTA29500.10585021584473675No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC29050.10423555153524076No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC28000.10046800147975013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)