FastQCFastQC Report
Wed 8 May 2024
EGAF00007878625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801591
Total Bases282.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC117890.4207966116396005No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC116180.41469293697759596Illumina Single End PCR Primer 1 (96% over 32bp)
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC84430.3013644746859909No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT83550.2982234023453102No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC73290.2616013543732829No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG62680.22373001626575756No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT55630.19856574353644055No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC55000.19631702129254414No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG51450.1836456499182072No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT46820.1671193261257621No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC46620.16640544604833468No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCG44310.15816013115404784No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC41560.1483442800894206No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC40110.14316864952807173No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT39600.14134825533063175No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG39390.14059868124933297No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC39330.14038451722610473No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT39060.13942077912157771No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG38670.13802871297059421No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC38660.13799301896672284No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT37170.13267461238988845No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG37000.13206781432407513No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG36910.1317465682892328No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA36400.12992617409179286No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC36160.12906951799887992No Hit
TTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTA35630.1271777357936972No Hit
CCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCC31110.11104404604383725No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT30860.11015169594705293No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC30040.10722478762960047No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC29540.10544008743603188No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC29140.10401232728117701No Hit
TGAACTCCTCACACCCAATTGGACCAATCTATTACCCTATAGAAGAACTA28690.10240609710696529No Hit
AAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCT28650.1022633210914798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)