FastQCFastQC Report
Mon 22 May 2023
EGAF00007878865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454331
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC129380.3745443039477108Illumina Single End PCR Primer 1 (96% over 31bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC66540.19262774760148926No Hit
TCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAAC54360.1573676639557703No Hit
GACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTA53260.15418325574474478No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC51480.1490303042759944No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT47180.1365821630874401No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC43750.12665259930215142No Hit
CGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCT42490.12300500444224945No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC40130.11617300137132197No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG38310.11090425324035247No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC35260.10207475774614534No Hit
CCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGC35160.10178526609059757No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG35060.1014957744350498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)