FastQCFastQC Report
Wed 8 May 2024
EGAF00007878904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294318
Total Bases332.7 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC375921.1411163099615762No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG243890.7403353288905321No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC241880.7342339142729998No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG135800.41222492789099296TruSeq Adapter, Index 6 (97% over 35bp)
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC123970.3763146120077054No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA106060.32194827578879753No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC97360.2955391677427619No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA92900.2820007054570931No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC88260.26791584783254074No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT80710.2449975988960386No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC73410.22283823237465236No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC71460.2169189495367478No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT70190.21306382686795872No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC69820.21194068089358709No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA67570.20511073915754338No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG66530.20195378831066096No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT64530.19588272898973325No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT63590.1930293311088972No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG60640.18407451861052881No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG58830.1785802099250892No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC56570.17171991289244087No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55720.16913971268104658No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG54770.16625595950360592No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG53800.16331149573295595No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT53640.16282581098728172No Hit
GAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAG52530.15945637306416685No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT50040.15189790420961183No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT49890.15144257476054224No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT47750.14494654128714957No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG46970.14257882815198775No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC45730.13881477137301257No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA45240.13732736183938526No Hit
CGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCA44190.1341400556958982No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG43900.1332597520943637No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA42080.12773508811231946No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC40910.12418351840957673No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC37570.11404484934362741No Hit
TCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGC37490.1138020069707903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37100.1126181504032094No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG36960.11219317625074446No Hit
TACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG36880.11195033387790736No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT36860.11188962328469806No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC36770.11161642561525634No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC35460.10763988176004866No Hit
GAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC35250.10700242053135124No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA34490.10469541798939871No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC34340.10424008854032915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)