FastQCFastQC Report
Wed 31 May 2023
EGAF00007878935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007878935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3774454
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC206100.5460392417022435Illumina Single End PCR Primer 1 (96% over 31bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC68140.1805294222687573No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC54690.1448951292027933No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC47440.12568705301482017No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG45750.12120958422065814No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC45180.1196994320238106No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT43380.11493053034955521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)