FastQCFastQC Report
Fri 10 May 2024
EGAF00007879221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007879221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4782352
Total Bases358.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG306020.6398943448746558No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT298950.6251108241300515No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG199330.4168032800596861No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG190200.39771225539232574No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA182150.3808795337524298No Hit
GTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGG148530.3105793969159945No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT137480.28747361131091986No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG110470.23099512541109476No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT106450.2225892196977554No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT106040.22173190095584766No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC102410.21414149355798148No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG102340.21399512206546067No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA100510.2101685530467017No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT98750.20648835552046357No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC97870.2046482567573445No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA97370.20360274609648138No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC95410.19950434430589803No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC91830.19201848797411816No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA87430.18281799415852282No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG87040.18200249584304962No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA84540.17677494253873408No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA83670.17495575398883229No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG80470.16826448575930839No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC76680.16033951494996604No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC76480.1599213106856208No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA76160.1592521838626684No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC69670.1456814554846653No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGC68650.14354861373650454No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA66610.13928293024018307No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT65720.13742192126384675No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT65520.1370037169995015No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT64320.13449449141343003No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG63390.13254984158422467No Hit
TCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGAT61800.12922511768268No Hit
CGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCT61640.1288905542712038No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG61030.1276150312649508No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA60090.12564947122252818No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGC60040.12554492015644184No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC59880.12521035674496567No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG59130.12364209075367101No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG59030.12343298862149837No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT56470.11807997403787927No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT55870.11682536124484355No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT55840.11676263060519175No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT55610.11628169570119472No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT55600.11626078548797746No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA55410.11586349143684947No Hit
CCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAG53410.11168144879339706No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG53390.11163962836696253No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA53040.11090777090435835No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGC52980.11078230962505478No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA51950.10862855766367678No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG51370.10741576529707558No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT51160.10697665081951307No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA51060.10676754868734045No Hit
CCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCG50550.10570112781326008No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGG50000.10455106608631068No Hit
CTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC49600.10371465755762017No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA49570.1036519269179684No Hit
GTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGG49540.1035891962783166No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT49410.10331736350649219No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT49150.1027736979628434No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT48920.10229276305884637No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT48510.1014354443169386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)