FastQCFastQC Report
Wed 8 May 2024
EGAF00007880084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132415
Total Bases159.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG141690.6644579033630883No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG137080.6428392221964299No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT130140.610293962479161No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA126340.5924737914524142No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA117080.5490488483714474No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC111560.5231627051957523No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT105750.49591660159959483No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT104180.4885540572543337No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT92190.4323267281462567No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA90190.42294769076375843No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC71720.33633228053638714No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA59240.2778070872695981No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG57130.26791220283106243No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC56560.2652391771770504No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC55370.25965864993446397No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA54630.25618840610293964No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG54560.2558601397945522No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT54500.2555787686730772No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT53440.2506078788603532No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG49720.23316286932890642No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC48870.22917677844134465No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT48060.2253782683014329No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG46370.2174529817132219No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG41570.19494329199522606No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT41560.19489639680831358No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC39640.18589252092111525No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT36360.17051089961381813No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT35340.16572759054874403No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG34140.1601001681192451No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC32780.15372242269914627No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC30980.14528128905489784No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA30950.1451406034941604No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT29900.1402166088683488No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG29800.1397476569992239No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC29600.13880975326097406No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA29140.13665257466299946No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA28550.13388575863516247No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT28350.13294785489691266No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG28220.13233821746705027No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA27420.128586602514051No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT27330.12816454583183856No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC27140.12727353728050123No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA26950.12638252872916386No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA26650.12497567312178914No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT26440.12399087419662683No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT26160.12267780896307706No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG26010.1219743811593897No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA24720.11592490204767834No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT23840.11179812559937911No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT23780.11151675447790416No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT23770.11146985929099167No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT23570.11053195555274184No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC23410.10978163256214198No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT23110.10837477695476724No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG22840.10710860690812998No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC22640.10617070316988016No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT22220.10420110531955552No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA22170.10396662938499308No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG22130.10377904863734311No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG22120.10373215345043062No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC21500.10082465186185614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)