FastQCFastQC Report
Wed 31 May 2023
EGAF00007880143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458406
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC189780.5487499154234639Illumina Single End PCR Primer 1 (96% over 32bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC86370.24973933077839908No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC48060.13896575474365935No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC44480.12861416502284578No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.12259983356494293No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC41160.11901436673427007No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT40280.11646984188669579No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC39480.11415663747981006No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT37570.10863386195837041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)