FastQCFastQC Report
Wed 8 May 2024
EGAF00007880153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3185325
Total Bases321.7 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC137660.43216940186637154Illumina Single End PCR Primer 1 (96% over 32bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC75120.2358315085587813No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC46850.14708075314135918No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC40180.1261409746258231No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC40020.12563867109321655No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC36380.11421126572641724No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT36340.1140856898432656No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT32500.1020304050607081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)