FastQCFastQC Report
Wed 31 May 2023
EGAF00007880199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3409135
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC208420.6113574264439513Illumina Single End PCR Primer 1 (96% over 31bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC72410.21239991962770616No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC62160.18233364181823247No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC49330.1446994618869596No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC49020.14379014031418527No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT47780.14015285402308797No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG41890.12287574414037578No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC39590.11612916472946949No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG37090.10879592623935397No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC35890.10527597176409852No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT34930.10246000818389416No Hit
GACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTA34840.10219601159825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)