FastQCFastQC Report
Thu 9 May 2024
EGAF00007880238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066155
Total Bases309.6 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC394361.2861711165939098No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG268450.8755265144782309No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC262520.856186331088937No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG226730.739460333870923TruSeq Adapter, Index 6 (97% over 35bp)
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC133020.4338332537004815No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT120470.39290251145163896No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC109640.35758140080980905No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC103180.33651266814626135No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA101870.3322402161665017No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA95810.312476048992957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90130.29395121903491506No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT85620.27924224313513174No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT85270.2781007483313792No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC82920.27043642607761187No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC78740.256803716707081No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC76720.2502156609825661No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG75030.24470387178730366No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG74910.24431250214030278No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG74680.24356237698355104No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA72760.23730046263153692No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG69060.2252332318490096No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT67380.21975405679099722No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT65940.21505762102698658No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG62380.2034469881659603No Hit
GAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAG61400.2002508027154531No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC60300.19666324761794496No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG58470.19069486050118145No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG58040.18929245259942826No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT54100.1764424825228992No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT53180.1734419818958924No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT52570.1714525195236379No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT52160.1701153398963849No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC50630.1651253768971236No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49880.16267931660336807No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA49780.1623531752308673No Hit
CGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCA49250.16062462595661342No Hit
GGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC48880.1594179028783607No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA48610.1585373211726087No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC46950.15312337438909643No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC46170.15057947168359068No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA44760.14598087833133028No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA44710.1458178076450799No Hit
GGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC43140.14069738809681834No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC43020.14030601844981744No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC42930.14001249121456677No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG41110.13407671823505335No Hit
TACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG39460.12869538558879118No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT39450.12866277145154112No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG37620.1226943843347776No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC37480.12223778641327655No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC37300.12165073194277524No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC36730.11979172611952103No Hit
TCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGC36600.11936774233527007No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC36500.11904160096276933No Hit
TGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG35430.11555188827701143No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG35100.114475621747759No Hit
GAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC34690.11313844212050599No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC34600.1128449148852553No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT34030.11098590906200109No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC34000.11088806665025087No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGG33210.10831154980749506No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC32800.10697437018024204No Hit
TGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTG32600.10632208743524055No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG31080.10136473857322935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)