FastQCFastQC Report
Mon 22 May 2023
EGAF00007880275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908428
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC286970.9866842156656449Illumina Single End PCR Primer 1 (96% over 33bp)
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG99600.3424530364856892No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC86850.29861492187532235No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC79680.27396242918855135No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT67010.2303993772580927No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC65090.22379787294029627No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC57500.19770130118400728No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG53190.1828822993039539No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG52310.17985660982496385No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC52080.17906580462022784No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48730.1675475548990726No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT46970.16149617594109256No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT43550.1497372463750177No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG42950.14767427627570634No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT42240.1452330949915212No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC41800.14372025025202617No Hit
GGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA41420.1424137025224623No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC40430.13900980185859854No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC36600.12584117605799422No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG36050.12395012013362545No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG34730.11941158591514041No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA34180.11752052999077164No Hit
GGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAA32690.11239748757748173No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT32500.11174421371269978No Hit
GTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAA32370.11129723685784898No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT32340.11119408835288341No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA31180.10720567949421475No Hit
CGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAG29620.10184195723600516No Hit
TCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCG29430.1011886833712232No Hit
GTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGA29270.10063855801140686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)