FastQCFastQC Report
Mon 22 May 2023
EGAF00007880435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388672
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC346091.0213145444587142Illumina Single End PCR Primer 1 (96% over 31bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC88780.261990537886228No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC77910.22991307509254363No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT67940.200491519981869No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC66240.19547480546951726No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG65350.1928484078718743No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC53280.15722973483417693No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC51360.15156379844375614No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG50480.14896691093147996No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT46070.1359529632847322No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41840.12347019717458638No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT41810.12338166691848605No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG39970.11795181121099947No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC39200.1156795346377578No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT38130.11252195550351288No Hit
GGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA38070.11234489499131223No Hit
TCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCG37820.11160714285714285No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC37060.10936437636926796No Hit
GTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAA36280.10706258971065952No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG36250.10697405945455918No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA35060.10346235929591296No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC35020.10334431895444587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)