FastQCFastQC Report
Wed 8 May 2024
EGAF00007880716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493768
Total Bases352.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG242140.693062618926042TruSeq Adapter, Index 27 (97% over 38bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC150660.4312249697175084No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG108210.30972291233991495No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC106940.30608786845606234No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG105940.3032256291774382No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC51980.14877919770288126No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT42890.1227614426601881No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA42140.12061476320122No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA38380.10985274351359335No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC37730.10799228798248767No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT36510.10450035606256625No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG36320.10395653059962769No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.10318372599439918No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC35320.10109429132100357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)