FastQCFastQC Report
Thu 9 May 2024
EGAF00007880733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007880733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310281
Total Bases334.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC198590.5999188588521639Illumina Single End PCR Primer 1 (96% over 33bp)
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC55260.16693446870522471No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC49270.14883932814162906No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT41960.12675661069256658No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC40360.12192318416472801No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC37190.11234695785644784No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG35730.10793645614979513No Hit
GACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTA33980.10264989588497168No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT33330.10068631635803728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)