FastQCFastQC Report
Wed 8 May 2024
EGAF00007881208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007881208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726912
Total Bases129.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA47310.2739572138012823No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC47160.2730886113478857No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT46320.26822443760886483No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC45320.262433754586221No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA41920.24274543230923176No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG41030.2375917244190787No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT38970.2256629173924323No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT37290.21593456991439056No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA36630.21211271911944557No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA36150.2093331912685765No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG36010.20852249564540637No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT34640.20058925990438423No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG34610.2004155394137049No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT34560.20012600526257274No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA29150.1687984101100693No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG28220.1634130748990105No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG28130.16289191342697254No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA26410.15293193862802507No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG25920.15009450394692955No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG25910.1500365971167031No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG25310.1465621873031168No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT24890.14413010043360636No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA24610.14250870918726605No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG24130.13972918133639697No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC23260.1346912871066968No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG22680.13133269095356334No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT22610.1309273431419783No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT22460.1300587406885817No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG22150.1282636289515621No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT21460.12426805766593782No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT21140.12241503909869178No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA21100.12218341177778602No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC20140.11662435607604787No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA19670.11390273505540525No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG19570.11332366675314087No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT19370.11216553014861208No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA19260.11152855501612126No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC18950.10973344327910164No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT18780.10874902716525219No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC18560.10747507690027054No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG18290.10591159248415669No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG18270.1057957788237038No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT18260.10573787199347738No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC18190.1053325241818923No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA18120.10492717637030724No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT17980.10411648074713709No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA17760.10284253048215544No Hit
CCTTAGACAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG17670.10232136901011749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)