FastQCFastQC Report
Wed 8 May 2024
EGAF00007882061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007882061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3984335
Total Bases402.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC133190.33428414026431014Illumina Single End Sequencing Primer (100% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC64190.16110593110268087No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG51640.12960757566821063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)