FastQCFastQC Report
Wed 8 May 2024
EGAF00007883566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007883566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011097
Total Bases304.1 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT382041.2687734735878653No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT233970.7770257816337368No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT198740.6600252333285842No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG196670.6531506623665727No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT194220.6450140928704721No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA179220.595198361261693No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC166430.552722147443274No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA164090.5449508933123045No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG162200.5386741111295983No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA134500.44668106009205283No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT126940.42157393136122817No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC126660.42064403770453096No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG125490.4167584106390462No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC110780.36790578317470346No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT110540.367108731468963No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC110290.3662784692754833No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT93960.31204574279739244No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG88570.29414528990597116No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA88240.293049343810578No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG81190.26963594995445184No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA64150.21304527884687874No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT63850.21204896421470315No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG63210.20992349299939522No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC62330.20700097007834684No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG62110.20627033934808475No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT59300.19693819229337348No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT59000.1959418776611979No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC51590.171332906246461No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC51500.17103401185680833No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG50910.16907459308019634No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG49630.16482365064958054No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT49060.16293065284844693No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG48930.16249891650783752No Hit
CACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC48430.16083839212087822No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA47940.1592110782216581No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG46590.15472766237686797No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC46040.15290108555121273No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA45670.15167229750486286No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC45240.15024424653207785No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC44570.14801914385355236No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG44330.1472220921478119No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT43810.14549514678537423No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT43730.14522946288346075No Hit
CACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTC43400.1441335167880676No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA41510.13785673460536144No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA40600.13483458022109548No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA39940.13264268803030924No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC38620.12825890364873666No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT38590.1281592721855191No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT38540.12799321974682318No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG38360.12739543096751782No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC37640.12500427585029641No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG37310.12390832975490328No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG36970.12277917317177094No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC36740.12201533195376968No Hit
CCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG36740.12201533195376968No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT34940.11603744416071617No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG34550.11474223513888793No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT33570.11148760734044769No Hit
CCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT33130.11002634587992349No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT33100.10992671441670593No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT32900.10926250466192222No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA32810.10896361027226953No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT32590.10823297954200746No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA32520.10800050612783313No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG32500.10793408515235477No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG31810.10564256149835094No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG31810.10564256149835094No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT31760.105476509059655No Hit
CCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGAT31330.10404845808687001No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA31170.10351709028304301No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT30990.10291930150373768No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT30810.10232151272443232No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC30670.10185656589608372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)