FastQCFastQC Report
Thu 9 May 2024
EGAF00007883922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007883922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713199
Total Bases375 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG214820.5785308032238509TruSeq Adapter, Index 7 (97% over 34bp)
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT101330.2728913801818863No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG81770.22021442966024712No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT71030.19129058259468454No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG63270.17039216050634506No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA58860.15851560877830678No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT57510.1548799296778869No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC56510.15218683404794625No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT45930.12369388228317414No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC45570.12272436785639552No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC42790.11523756200516051No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG41810.11259832828781867No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT39840.1072929298968356No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA38050.10247228871924181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)