FastQCFastQC Report
Fri 10 May 2024
EGAF00007883945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007883945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3694521
Total Bases373.1 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC236470.6400559098188913Illumina Single End PCR Primer 1 (96% over 32bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC64570.17477231825181128No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC41890.1133841166419138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)