FastQCFastQC Report
Wed 8 May 2024
EGAF00007883974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007883974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886063
Total Bases291.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG298711.0350085912885478TruSeq Adapter, Index 27 (97% over 38bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC110660.383428913367449No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG85670.2968403669635763No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG85640.29673641912875776No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC83550.2894947199697304No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT46340.16056475551642496No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC40100.13894360587416144No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG39770.13780017969115713No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT39070.13537473021205704No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC32090.11118953397760202No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT30840.10685837419349474No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA30060.1041557304882118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)